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Poster Index
THEME A POSTERS: REPLACEMENT AND REDUCTION ALTERNATIVES
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A1: Involvement of Heat Shock Proteins and P-Glycoprotein in CdCl2-Induced Toxicity and Intestinal Barrier Function
Monica Boveri, Alessandra Gennari, Patricia Pazos, and Pilar Prieto
A2: Expanding the Application of the Embryonic Stem Cell Test (EST) by Using Tissue-Specific Molecular Markers
R. Buesen, A. Seiler, A. Visan, I. Pohl, E. Genschow, and H. Spielmann.
A3: Comparative Assessment of the Acute Skin Irritation Potential of Detergent Formulations Using a Novel Human Four-hour Patch Test Method
P. Casterton, F.H. Kruszewski, M. Robinson, N.K. Snyder, M.E. Blazka, F. Heitfeld, J.E. Swanson, D. Mallon, and R. Gingell.
A4: Co-culture of Sensory Neural Cells with Human Corneal Epithelium
R. Clothier, P. Chapman, H. el Haroun, H. Cox, and R. Budworth.
A5: EPISKINTM as a Product Development Tool: Comparison of In Vitro (Reconstructed Epidermis) and In Vivo (Human) Skin Responses to Irritation Potential of Cationic Surfactants
J. Cotovio, E. Combrier , B. Hall, and C. Van Den Berghe.
A6: Characterization of Genetically Engineered HCYP2D6*1 Cell Lines (and Mock Transfected Control) in Serum-Free Medium
L. Raimondo Csiky, J. De Graeve, A. Bogni, and S. Coecke.
A7: In Vitro Corneal Recovery Assay Using a Reconstituted Human Corneal Epithelial Model
B. De Wever, C. Tornier, C. Rosdy, and R. Beuerman.
A8: Tissue-Specific Toxicity of Forty Chemopreventive Agents in Cell Cultures from Normal Human Tissues
E. Elmore, S. Siddiqui, N. Desai, T.-T. Luc, V.E. Steele, and J.L. Redpath.
A9: Correlation of Difluoromethylornithine Efficacy and Biomarker Response in the Human Epidermal Cell Assay with Clinical Trial Biomarker Modulation
E. Elmore, D. Stringer, V.E. Steele, E.W. Gerner, and J.L. Redpath.
A10: Evaluation of a Human Red Blood Cell Lysis Test for Predicting the Eye Irritancy of Industrial Enzyme Preparations
S.G. Elvig and N. Berg.
A11: Hypochlorite-Anion as a Protector in Methylmethacrilate Cytotoxicity
E. Eropkina, M. Eropkin, E. Mamaeva, and V. Mashkov.
A12: A Preliminary Study for the Determination of Photohemolitic Potential of Five National Production Perfumes, Using the 81's Invittox Protocol
Gaston Garcia Simon, L. Dios Oliva, and A. Valdivieso Garcia.
A13: Detection of Endotoxins and Other Pyrogens by the In Vitro Pyrogen Test (IPT) as an Alternative to Rabbit Experiments
T. Hartung, S. Fennrich, S. Schindler, I. Kindinger, S. Reichstein, S. Hofmann, and A. Wendel.
A14: Replacement of Animal Tests for Cyto- and Genotoxicity by Recombinant Mammalian Cells
C.E. Hellweg, C. Baumstark-Khan, A. Arenz, and G. Horneck.
A15: Evaluation of CD86 and Mhc Class II Molecule Expression on THP-1 Human Monocyte Cells as Predictive Endpoint for Contact Sensitizers
Hiroshi Itagaki, Masatoshi Hoya, Takao Ashikaga, and Hidekazu Toyoda.
A16: The Third Frame Toxicity Committee, A Strategy for Implementing Alternatives in Toxicity Testing
M. Jackson, M. Balls, L. Bansil, M. Barratt, D. Bell, P. Botham, C. Broadhead, R. Clothier, R. Combes, E. George, J. Fentem, I. Indans, G. Loizu, V. Navaratnam, V. Pentreath, B. Phillips, H. Stemplewski, and J. Stewart.
A17: Factors Effecting the Attachment and Penetration of Schistosome mansoni Larvae in Response to a Human Epidermal Model
N. Khammo, A. Bartlett, R.H. Clothier, and P.J. Whitfield.
A18: EpiAirway, Differentiated Airway Epithelial Cultures for Pre-clinical Respiratory Studies
M. Klausner, J. Kubilus, P. Hayden, R. Jackson, J. Sheasgreen, E. Roemer.
A19: Full Thickness EpidermTM: A Dermal-Epidermal Skin Model to Study Epithelial-Mesenchymal Interactions
M. Klausner, J. Sheasgreen, J. Kubilus, S. Ayehunie, P. Hayden, S. Lamore,
and K. Bellavance.
A20: Measurement of Antibody Responses Against E Coli F4 Antigens in Sows after Vaccination as an Approach to Replace Efficacy Challenge Procedures in Newborn Piglets
M. Krug, D. Hausleithner, R. Taddey, B. Kobe, and K. Cussler.
A21: In Vitro Quantitative Determination of Ophthalmic Irritability by Chorioallantoic Membrane Test with Tryptan Blue Staining as Alternative to Eye Irritation Test
A. Lagarto, R. Vega, I. Guerra, and R. Silva.
A22: Comparative Study of Red Blood Cell Assay in Rat and Calf Blood as Alternatives of Draize's Eye Irritation Test
A. Lagarto, R. Vega, I. Guerra, and Y. Vega.
A23: Prediction of Acute Toxicity by In Vitro Assays in Drug Discovery
A. Martinez, E. Herreros, and D. Gargallo-Viola.
A24: Toxic Effects of Ketamine Hydrochloride (VetalarTMV) on Cultures of Marmoset Bladder Epithelial Cells and Whole Rat Bladders In Vitro
D.G. Nemitz, C. Westmoreland, E. George, J. Klapwijk, and N. Watts.
A25: Biomimetic Extraction: An Effective In Vitro Tool For Determining the Aquatic Toxicity of Complex Petroleum Products
D.R. Peterson, T.F. Parkerton, D.J. Letinski, and E.J. Febbo.
A26: Toxicity of Phenethylisothiocyanate and Dithiothreitol on Fa32 Cells and their Effect on Glutahione S-Transferase
E.M. Scheers and P.J. Dierickx.
A27: Gingival Tissue Model for Prediction of Irritancy of Oral Care Products
J. Sheasgreen, M. Klausner, J. Kubilus, and B. Breyfogle.
A28: Estimation Method of ET50 Scores for Three-dimensional Human Skin Models
I. Sonoda, H. Kojima, and I. Yoshimura.
A29: Drug-Drug Interactions Between Antiarrhythmic Drugs in Chick Embryos
T. Sugiyama, Y. Yoshiyama, and K. Tsuchimoto.
A30: Development of Rat Skin Transcutaneous Electrical Resistance Test for Skin Corrosion Testing of Different Substances
Raiza Vega, Isbel Guerra, Yamilet Vega, Alfredo Iglesias, and José Roche.
A31: CAM-TBS Assay to Predict the Eye Irritation of Arginine-based Dimeric Surfactants
M.P. Vinardell, M. Mitjans, C. Moran, L. Perez, E. Piera, P. Clapes, and M.R. Infante.
A32: Growth of Human Epithelial and Stromal Fibroblast Cells in Serum-Free Media
S.F.Webb.
A33: In Vitro Cytotoxicity of Benzalkonium Chloride on Sv40 Transformed Human Corneal Epithelial Cells (HCE-T)
Ann M. Wright and Mary Mowrey-Mckee.
A34: In Vitro Pig Heart Perfusion--A Powerful Tool in Research and Development
B. Christ, I. Ast, and D. Heydeck.
A35: In Vitro Approach to Determine the Bioavailability of Topically Applied Cationic Hair Dyes
W. Steiling.
THEME B POSTERS: REFINEMENT AND REDUCTION ALTERNATIVES
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B1: Fish as an Alternative Model to Mammals for Genotoxicity Research
S.P. Bhunya and S.N. Sahoo.
B2: Optimizing Resources via Reduction: The FRAME Reduction Committee
R. Combes , S. Vaughan, and M. Festing.
B3: Environmental Enrichment: Beneficial Effects in a Rodent Model of Lead Neurotoxicity
Tomas R. Guilarte, Christopher D. Toscano, Jennifer L. McGlothan, and Shelley A. Weaver.
B4: Murine Models of Tobacco Smoke-Induced Pulmonary Inflammation and Emphysema
H. McMath, P. Thornton, D. Spicer, C.F. Fox, J. Weedon, and M.F. Fitzgerald.
B5: Experimental Design: Computer Simulation for Improving the Precision of an Experiment
H. van Wilgenburg, I. Krulichova, and P.G. van Schaick Zillesen.
THEME C POSTERS: POLICY AND ETHICS
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C1: Policymakers Support Non-animal Alternative Methods: An Overview of New Laws in the United States
Sara J. Amundson.
C2: Welfare Implications of Different Approaches to Investigate Gene Function and Regulation
R.D. Combes and E. Jenkins.
C3: The Alternatives Toxicological Group in Cuba--Developments and Perspectives
Gaston Garcia Simon.
C4: IACUC's and Ethics
Lise J. Houde, Claude Dumas, and Therese Leroux.
C5: A Handbook for Lay Members of Local Ethical Review Processes
Maggy Jennings and Jane A. Smith.
C6: Implementation of EU Directive on Animal Experimentation at the University of Barcelona: Role of the Ethical Committee
Carme Navarro and Miquel Moretó.
C7: Xenotransplantation--Costs Versus Benefits
Irmela W. Ruhdel.
C8: The Work of Schweizer Tierschutz STS (Swiss Animal Protection, SAP) in the Area of Laboratory Animal Welfare and Experimentation
Norma Schenkel and Eva Waiblinger.
C9: History of The Humane Society of the United States' Work Related to Animal Research
M.L. Stephens.
C10: The 'Problem R'--Replacement
M.B. Sweetland, T.K. Seidle, and J.T. Sandler.
C11: The Regulation of Animal Experimentation in Lithuania
S. Uleckiene.
C12: Animal Experiments Outside the Laboratory Door: Different Norms
and Values?
Katinka Waelbers, Tjard de Cock Buning, and Jan Vorstenbosch.
THEME D POSTERS: EDUCATION AND INFORMATION
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D1: An Alternative Model for Laboratory Surgical Training
Emad Aboud.
D2: Nobody Knows: A Proposal for Data Collection as a Necessary Condition for Evidence-based Ethical Review
N.E. Cohen.
D3: The European Resource Centre for Alternatives in HE (EURCA: http://www.eurca.org)
J. de Boo, J. van der Valk, and D. Dewhurst.
D4: A New Computer-Aided Learning (Cal) Program to Teach Better Experimental Design
D. Dewhurst, M.F.W. Festing, and J. Broadhurst.
D5: ZEBET Database on Alternatives to Animal Experiments on the Internet
B. Grune, A. Doerendahl, D. Koehler-Hahn, R. Box, H. Wohlgemuth, and H. Spielmann.
D6: National Library of Medicine's Alternatives to Animal Testing Database on the Web
V.W. Hudson, F. Chang , and B. Mashayekhi.
D7: Conscientious Objection and the Role of Students in Curricular Change
N.D. Jukes.
D8: Are School Science Competition Rules Fair to Animals?
Leslie A. King.
D9: The Application of Alternatives, Addressing Difficulties that Arise
T. Kutsenko, M. Makarchuk, and S. Danilov.
D10: The Use of Alternative Programs in Training the Students of Pedagogical University
A.A. Pinskiy and L.P. Starikova.
D11: The Databases of the ECVAM-SIS Online
A. Roi, A. Burke, E. Sedin, and M. Balls.
D12: The ECVAM Thesaurus of Advanced Alternative Methods (TAAM) to Animal Experiments
A. Roi, S. J. Nelson, K. Bottrill , B. Grune, and M. Balls.
D13: Technology and Innovation: Creating the Ultimate Alternative for Non-invasive Dissection
R.E. Schneider and Dennis E. Morse.
D14: Status of Laboratory Animal Science in Lithuania
V. Simkeviciene and O. Ruksenas.
D15: Muscle Control, A Realistic Practical Course On Computer
S. van Duin and N.W. Meijer.
THEME E POSTERS: TEST DEVELOPMENT, VALIDATION AND IMPLEMENTATION
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E1: The Evaluation of an Alternative Mucosal Irritation Test Using Slugs
E. Adriaens and J.P. Remon.
E2: Study of Alternatives to Testing for Endocrine Disrupters in Rat Whole Embryo Culturetes
Masaharu Akita, Atsushi Yokoyama, and Yukiaki Kuroda.
E3: Percutaneous Absorption of Mexoryl SXTM in Human Volunteers: Comparison with In Vitro Data
F. Benech-Kieffer, W.J.A. Meuling, C. LeClerc, L. Roza, J. LeClaire, and G. Nohynek.
E4: The Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) Peer Review Panel Evaluation of the Revised Up-and-Down Procedure (UDP) for Acute Oral Toxicity
B.C. Blackard, R.R. Tice, W.S. Stokes, and R.N. Hill.
E5: Development of an In Vitro Test Battery for the Estimation of Acute Human Systemic Toxicity: Presentation of the EDIT Project
C. Clemedson.
E6: Episkinª as a Product Development Tool: Comparison of In Vitro (Reconstructed Epidermis) and In Vivo (Human) Skin Responses to Irritation Potential of Cationic Surfactants
J. Cotovio, E. Combrier, B. Hall, and C. Van Den Berghe.
E7: An Improved In Vitro Protocol to Assess Ocular Irritating Potential of Chemicals
C. Ebenhahn, A.M. Verda, J.N. Pierre, C. Van Den Berghe, and M. Mariani.
E8: Evaluation of a Human Red Blood Cell Lysis Test for Predicting the Eye Irritancy of Industrial Enzyme Preparations
S. G. Elvig and N. Berg.
E9: Application of a Genetically Engineered PC12 Cell Line as Part of an In Vitro Integrated Testing Strategy for Detecting Neurotoxic Effects
J. Gartlon, C. Eskes, D. Sladowski, E. van Vliet,, R. Clothier, and S. Coecke.
E10: Comparison Between Permanent Female and Male Egc-Lines of Balb/cJ Mice for Reproductive Toxicity Testing
E. Genschow, M. Klemm, C. Barrabas, A. Seiler, and H. Spielmann.
E11: Inflammatory Response to Pyrogens Absorbed to Biomaterials Determined by a Novel Whole Blood Assay Without Animal Use
Thomas Hartung, Stefan Fennrich, Karin Kullmann, Silvia Reichstein, and Albrecht Wendel.
E12: Whole Blood Cytokine Response as a Measure of Airborne Microbial Contamination
Thomas Hartung, Ilona Kindinger, Stefan Fennich, Johannes Baur, Bert Zucker,
and Gunther Linsel.
E13: In Vitro Methods for Assessing the Safety and Toxicity of Australian Essential Oils
A.J. Hayes and B. Markovic.
E14: In Vitro Oral Irritation Assessment of Dental Formulations Using the Skinethicª Human Oral Epithelium Model
P.A. Jones, A.V. King, and V.A. Baker.
E15: Effects of Nicotine in Genetically Engineered PC12 Cells Expressing
p53 and bcl-2
A. Kinsner, L. Costa, and S. Coecke.
E16: EpivaginalTM, a Human Tissue Model for Vaginal Irritation Studies
M. Klausner, J. Kubilus, C. Cannon, J. Sheasgreen, and E. Lonardo.
E17: Effect of Chemicals on Squamous Differentiation in the Beas-2B Cell Line
J. Malton, A.C. Gray, and R.H. Clothier.
E18: Assessment of Chemically Induced Photogenotoxicity by Complementary In Vitro Approaches
L. Marrot, J.P. Belaidi, C. Chaubo, J.R. Meunier, P. Perez, and C. Agapakis-Causse.
E19: An In Vitro Method for Visualizing the Time Course of Cytotoxic Effects on the Corneal Epithelium
Steven Matsumoto, William Way, Kirk Tarlo, Brian Short, and Balbir Brar.
E20: A Tissue Engineered Conjunctiva-Sclera for In Vitro Toxicology Testing
Lea Muzakare and May Griffith.
E21: Three-step Method of Data Analysis, Incorporating Multiple Contrast Method to Detect Carcinogenic Substances in Cell Transformation Assay
H. Nishiyama, T. Tsuchiya, I. Yoshimura, M. Umeda, and collaboration with 19 laboratories.
E22: Development of an Assay Method for Predicting Tumor Promoters Using V-Ha-Ras-Transfected Balb/C 3T3 Cells (BHAS 42 Cells)
K. Ohmori, S. Asada, K. Sasaki, N. Tanaka, and M. Umeda.
E23: Establishment of LD50 Reference Values for Chemicals Used in Validation Studies of In Vitro Acute Toxicity Assays
M.W. Paris, J.A. Strickland, R.R. Tice, and W.S. Stokes.
E24: Refined Episkin Protocol for the Assessment of Acute Skin Irritation of Chemicals
P. Portes, M.H. Grandidier, C. Cohen, and R. Roguet.
E25: Assessment of Weak Phototoxic Potential of Chemicals Using Human Reconstituted Epidermis EPISKIN
P. Portes, M.J. Pygmalion, E. Popovic, and M. Mariani.
E26: Cell Tolerance Investigation of Four Commercial Multipurpose Contact Lens Solutions
Patrice Rat, Sébastien Perrot, and Jean-Michel Warnet.
E27: Integration of Alternative In Vitro Procedures for the Ecotoxicological Evaluation of Pentachlorophenol
G. Repetto, A. Jos, M.J. Hazen, J.C. Ríos, M.L. Molero, A. del Peso, M. Salguero,
P. Fern‡ndez-Freire, J.M. Pérez-Martín, M.C. Rodríguez-Vicente, and M. Repetto.
E28: An Alternative Strategy for the Assessment of Chemicals for Endocrine Disrupting Effects
T.K. Seidle, G. Langley, and R.W. Combes.
E29: A Reproducible Tissue Model for Ocular Irritancy Testing
J. Sheasgreen, M. Klausner, J. Kubilus, and J. Harbell.
E30: Validation Study Design to Evaluate In Vitro Cytotoxicity Assays for Predicting Rodent and Human Acute Systemic Toxicity
W.S. Stokes, M. Balls, J.A. Strickland, A. Worth, M.W. Paris, S. Casati, and R.R. Tice.
E31: Selection of Reference Chemicals for the Validation of In Vitro Cytotoxicity Assays for Predicting In Vivo Acute Systemic Toxicity
J.A. Strickland, M.W. Paris, R.R. Tice, W.S. Stokes, M. Balls, A. Worth, and S. Casati.
E32: Using the Bovine Corneal Opacity and Permeability (BCOP) Assay to Evaluate Ingredient Interactions Contributing to the Eye Irritation Potential of Products
J.E. Swanson and J.W. Harbell.
E33: Use of Daphnia magna as an Alternative Bio-object in Pharmacology and Toxicology
V.D. Tonkopii and A.O. Zagrebin.
E34: Development of a New Opacitometer for the Bovine Corneal Opacity and Permeability (Bcop) Assay
F. van Goethem, E. Hansen, M. Sysmans, and P. Vanparys.
E35: Characterization of Genetically Engineered PC12 Cell Lines for Detecting p53-Mediated Cell Death
E. van Vliet, C. Eskes, J. Gartlon, and S. Coecke.
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